Anti-Stat3 Antibody

Stat3 (Signal Transducer and Activator of Transcription 3) is a key member of the STAT family of cytoplasmic transcription factors that transmit signals from cell-surface receptors to the nucleus. This unconjugated recombinant rabbit monoclonal antibody from Antibodies.com is raised against the Stat3 target and supplied as a 100 µL liquid formulation in TBS (pH 7.4) with 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative.

The reagent is validated for western blot, immunocytochemistry/immunofluorescence, and immunohistochemistry workflows, supporting detection of endogenous Stat3 protein in fixed cells and tissue sections. It reacts with human, mouse, rat, and zebrafish samples, making it a flexible tool for cross-species studies spanning cancer cell line panels, primary rodent tissues, and developmental zebrafish models. As a monoclonal reagent, it offers lot-to-lot consistency for longitudinal signaling studies, while the recombinant rabbit backbone often gives cleaner backgrounds on rodent sections than typical mouse monoclonals.

Researchers use this clone to interrogate JAK-STAT pathway activity, profile constitutive Stat3 phosphorylation (Tyr705) in tumor lysates by western blot, and map nuclear versus cytoplasmic Stat3 localization by immunofluorescence or chromogenic IHC. Aliquoting upon first use is recommended to limit freeze-thaw cycles and preserve activity across long-running projects, and the unconjugated format lets users pair it with a secondary detection reagent of their choice.