Anti-Stat1 (phospho-S727) Antibody

Phosphorylation of Signal Transducer and Activator of Transcription 1 (Stat1) at serine 727 cooperates with tyrosine 701 phosphorylation to drive maximal transcriptional output in the JAK-STAT signaling cascade. This rabbit polyclonal antibody from Antibodies.com specifically recognizes the phospho-S727 form of Stat1, enabling researchers to monitor cytokine-, interferon-, and growth factor-driven activation states in their experimental systems.

Each vial contains 100 µl of unconjugated antibody at 1 mg/ml, prepared in phosphate buffered saline (PBS) at approximately pH 7.2 with 0.05% sodium azide added as a preservative. The reagent is validated for Western blot (WB) and immunohistochemistry (IHC) applications and has been confirmed to react with Human, Mouse, and Rat samples, supporting use across a broad spectrum of cell line and tissue-based immunology, virology, and signal transduction studies.

Store refrigerated in the original formulation to maintain reagent performance, and aliquot upon first use to minimize repeated freeze-thaw cycles. The polyclonal format provides multi-epitope recognition surrounding the phospho-S727 modification, which is particularly valuable when working with fixed tissue sections or whole-cell lysates to distinguish active versus resting Stat1 populations.