Anti-Stat1 (L177) Antibody

Signal Transducer and Activator of Transcription 1 (STAT1) is a 91 kDa latent cytoplasmic factor activated by Janus kinases downstream of type I and type II interferon receptors, where phosphorylation on tyrosine 701 and serine 727 drives dimerization, nuclear translocation, and induction of interferon-stimulated genes involved in antiviral defense and immune cell maturation. Antibodies.com provides a rabbit polyclonal anti-STAT1 reagent raised against a synthetic peptide spanning the region around leucine 177 near the protein's N-terminus, supplied as 100 µl of liquid at 1 mg/ml in phosphate buffered saline (pH ~7.2) with 15 mM sodium azide as a preservative. The unconjugated primary antibody is validated for western blot and immunohistochemistry workflows and cross-reacts with human, mouse, and rat samples, supporting comparative studies of JAK-STAT signaling across model systems. Researchers apply this reagent to track STAT1 activation status in lysates and tissue sections, dissect cytokine-driven transcriptional programs, and characterize disease contexts in which STAT1 function is disrupted, including chronic mucocutaneous candidiasis, Mendelian susceptibility to mycobacterial disease, and STAT1 gain-of-function syndromes. Because the polyclonal format recognizes multiple epitopes on the target, the antibody also detects truncated STAT1 isoforms and can be paired with secondary detection reagents for chromogenic or chemiluminescent readouts in cell culture and ex vivo tissue samples.