Anti-SRF (N153) Antibody

Manufactured by Antibodies.com and distributed under the Bioworld Technology catalog code BS9121, this affinity-purified reagent is a polyclonal antibody raised in rabbit against serum response factor (SRF), generated using a synthetic peptide corresponding to amino acids 121-170 of the human protein. Each vial contains 50 µl of antibody formulated at approximately 1 mg/ml in phosphate buffered saline (PBS) supplemented with 0.05% sodium azide at pH 7.2, and the preparation is unconjugated with no fluorophore or enzyme tag attached. The antibody has been validated for Western blotting (WB) and immunohistochemistry (IHC) on human and mouse samples, where it specifically detects endogenous levels of SRF, a transcription factor that binds the serum response element (SRE) and serves as a constitutive element in many muscle-specific promoters. The IgG fraction was purified from rabbit antiserum by affinity chromatography using the epitope-specific immunogen and shows purity greater than 95% by SDS-PAGE, yielding a band at the expected molecular weight of approximately 67 kDa. For daily use the reagent should be kept at 4 °C for short-term storage, while long-term stability is maintained by aliquoting and storing at –20 °C, taking care to avoid repeated freeze–thaw cycles. Intended strictly for research applications and not for diagnostic or therapeutic use, this polyclonal reagent is supplied at 2030.0 SAR and provides a reliable tool for laboratories characterising SRF-dependent transcriptional responses in cell biology and signal-transduction studies.