Anti-SREBP-1 Antibody

For laboratories studying lipid metabolism and the transcriptional control of cholesterol biosynthesis, this unconjugated rabbit polyclonal reagent from Antibodies.com recognizes SREBP-1 (Sterol Regulatory Element-Binding Protein 1), a basic-helix-loop-helix-leucine zipper transcription factor that drives lipogenic gene expression. Supplied as 100 µl of antiserum, the antibody is validated for Western blot and ELISA workflows across Human, Mouse, and Rat samples.

Investigators commonly deploy this polyclonal reagent to resolve the precursor (~125 kDa) and cleaved nuclear (~68 kDa) SREBP-1 forms in liver, adipose, and hepatic cell line lysates. Its polyclonal profile broadens epitope coverage, which is helpful when tracking cleavage-dependent activation of the SREBP pathway under sterol-loaded or sterol-depleted culture conditions. For long-term integrity, store aliquots frozen, limit freeze-thaw cycles, and keep refrigerated working stocks; protect from light during extended bench use at room temperature.

Each vial is intended for research use only and supports immunoblotting at standard dilution ranges alongside direct or indirect ELISA formats where SREBP-1 abundance in tissue extracts is being quantified. The unconjugated format leaves reporter choice to the user, enabling pairing with HRP, biotin, or fluorophore-conjugated secondary antibodies as the experimental design demands.