Anti-SRC (phospho Y416) Antibody

The SRC kinase Y416 autophosphorylation site sits in the activation loop of the kinase domain, and phosphorylation at this residue is widely used as a read-out of SRC catalytic activity in cell signaling studies. This rabbit polyclonal antibody from Antibodies.com is raised against the phospho-Y416 epitope and detects the activated form of SRC in western blot workflows using human and mouse samples. The reagent is supplied as an unconjugated IgG in a 50 µL volume, ready to be paired with a secondary antibody of choice such as HRP- or fluorophore-conjugated anti-rabbit IgG. It is validated for western blot and reacts with both human and mouse SRC, supporting studies of SRC pathway activation in cancer research, signal transduction investigations, and phosphorylation mapping experiments.