Anti-SRC (phospho Y416) Antibody

Phospho-specific immunoblotting reagent from Antibodies.com for examining SRC activation in human and mouse samples. This rabbit polyclonal primary antibody recognizes SRC phosphorylated at tyrosine 416 and is supplied as an unconjugated IgG preparation in a 200 µl volume. It is listed for Western blot applications.

The unconjugated format allows researchers to select an appropriate anti-rabbit secondary antibody and chemiluminescent, fluorescent, or chromogenic detection system. Typical uses include comparing SRC Y416 phosphorylation across treated and untreated cell lysates or tissue samples, monitoring signaling responses, and investigating kinase-pathway activity.

For meaningful interpretation, phospho-SRC signal should be assessed alongside suitable stimulation controls, total SRC abundance, and a loading control. Antibody dilution, sample loading, incubation conditions, and exposure settings may require optimization according to the sample type and selected detection workflow.