Anti-Src (Ab-529) Antibody

A rabbit polyclonal reagent from Antibodies.com is raised against the proto-oncogene tyrosine-protein kinase Src and binds an epitope located around residue 529 of the protein sequence. Src is a 60 kDa non-receptor tyrosine kinase implicated in signal transduction networks that govern cell proliferation, adhesion, motility, and cytoskeletal organization, and is a common focus in cancer biology, neuroscience, and immunology research.

Supplied as 100 µl of unconjugated antisera, this primary antibody is validated for Western blot and immunohistochemistry workflows and shows reactivity against human, mouse, and rat samples. Polyclonal sera recognize multiple epitopes across the target, which is useful for monitoring total Src expression or studying upstream regulatory phosphorylation events in cell lysates and fixed tissue sections. Store at -20 °C in working aliquots to preserve antibody activity and limit freeze-thaw cycles of the stock solution.

The unconjugated format allows users to pair this reagent with their preferred anti-rabbit IgG secondary antibodies for chromogenic or fluorescence-based detection, and supports studies of receptor tyrosine kinase signaling, focal adhesion biology, and tumor cell migration on immunoblotting membranes and in tissue sections.