Anti-SPHK1 Antibody

From the Antibodies.com catalog of primary antibodies comes a rabbit polyclonal reagent raised against sphingosine kinase 1 (SPHK1), validated for Western blot (WB), immunohistochemistry (IHC) and immunofluorescence (IF). The affinity-purified antibody is supplied as a 50 µL liquid aliquot at approximately 1 mg/mL in PBS buffer containing 0.1% sodium azide and 50% glycerol, and is unconjugated, leaving the choice of label to the end user. The host species is rabbit, the clonality is polyclonal and the isotype is IgG, supporting detection of endogenous SPHK1 across human, mouse and rat samples. SPHK1 is detected at the expected molecular weight of around 43 kDa, making the reagent suitable for studies of sphingolipid signalling, cell-survival pathways and related cancer-research applications. For routine use, the antibody should be aliquoted and stored at –20 °C, with short-term handling at 4 °C, avoiding repeated freeze/thaw cycles to preserve activity. Each lot is purified by affinity-chromatography against an epitope-specific immunogen, yielding greater than 95% purity by SDS-PAGE. This format is intended for research use only and is not approved for diagnostic applications, making it a reliable choice for laboratories probing SPHK1 expression in cultured cells and tissue sections.