Anti-SPAG16 Antibody

Raised in rabbit against the ciliary and flagellar protein SPAG16, this unconjugated polyclonal IgG reagent from Antibodies.com is supplied as 200 µL per vial and has been validated for Western blot and immunohistochemistry on human, mouse, and rat samples. The immunogen corresponds to recombinant full-length human SPAG16, and the antibody has been affinity-purified to reduce background in chromogenic and fluorescence workflows.

SPAG16 (Sperm-Associated Antigen 16) supports axoneme assembly and central pair formation in motile cilia and sperm flagella, and the protein is widely investigated in the context of primary ciliary dyskinesia, male infertility, and developmental ciliopathies. Endogenous expression has been documented in testis, trachea, brain, oviduct, and select cultured cell lines, making the reagent useful for cross-tissue comparisons of ciliary architecture and isoform patterns. The polyclonal format recognizes multiple epitopes, which is helpful when probing full-length SPAG16 isoforms and proteolytic fragments generated from the locus.

Working aliquots are recommended to limit repeated freeze-thaw cycles of the stock; store refrigerated and protect from light between uses to preserve activity. Because the reagent is unconjugated, it can be paired with user-selected HRP, alkaline phosphatase, biotin, or fluorophore-conjugated secondaries for either chromogenic detection on Western blot membranes or immunofluorescence detection on fixed tissue sections.