Anti-SP-B Antibody

Surfactant Protein B (SP-B) is a small hydrophobic lipoprotein secreted by alveolar type II pneumocytes and Club cells, where it organizes the phospholipid film at the air-liquid interface of the lung and is indispensable for normal breathing mechanics at birth and throughout life. This unconjugated, affinity-purified reagent from Antibodies.com is a rabbit polyclonal antibody raised against SP-B and is validated for Western blot and ELISA workflows against human samples. Supplied at 100 µl per vial, the antibody recognizes the mature SP-B peptide and is widely used by respiratory researchers to probe surfactant biogenesis, neonatal respiratory distress syndrome (NRDS), pulmonary alveolar proteinosis, and inflammatory lung injury models. Because the target is highly hydrophobic and the dodecamer is processed from a larger pro-protein in the lamellar bodies of type II cells, immunoblots typically resolve a band near 8 kDa, with dimeric and oligomeric forms appearing at higher apparent molecular weight. The antibody is shipped refrigerated and should be stored at -20 °C in working aliquots to limit freeze-thaw cycles and preserve epitope recognition. Investigators working on surfactant biology, neonatal pulmonology, or comparative respiratory studies across mammalian models often pair this reagent with markers such as pro-SP-C or ABCA3 for phenotypic characterization of alveolar epithelium, and it is also applied in immunohistochemistry of lung tissue sections to confirm type II cell differentiation in stem-cell-derived or organoid culture systems.