Anti-SNRPD3 Antibody

This unconjugated polyclonal antibody from Antibodies.com targets SNRPD3, the 13 kDa Sm-D3 protein that forms a core component of the U4/U6.U5 tri-snRNP and the broader SMN complex responsible for assembling the small nuclear ribonucleoproteins that carry out pre-mRNA splicing. Raised in rabbit and supplied as 100 µl of a 1 mg/ml stock in phosphate buffered saline (PBS, pH 7.2) with 0.05% sodium azide, the reagent is validated for western blot detection of the endogenous antigen in human, mouse and rat samples.

SNRPD3 is one of the seven canonical Sm proteins that ring the snRNA in each spliceosomal snRNP, and changes in its expression have been examined in studies of spliceosome assembly, motor neuron survival models, and cancer cell lines where altered snRNP biogenesis has been reported. Researchers working on RNA processing machinery, spinal muscular atrophy models, or comparative studies of Sm-ring composition across tissues can use this reagent to probe steady-state expression levels and incorporation into the snRNP pool on western blot membranes.

For handling, store the vial refrigerated for short-term use or aliquot and freeze at -20°C for longer term; avoid repeated freeze-thaw cycles to preserve activity. Because the polyclonal format recognizes multiple epitopes on the Sm-D3 polypeptide, it is also useful when monitoring proteolytic fragments or post-translationally modified forms in research use only workflows.