Anti-SNRPD3 Antibody

SNRPD3 (Small Nuclear Ribonucleoprotein D3) is a core component of the spliceosomal snRNP complexes, essential for pre-mRNA splicing machinery assembly. This rabbit polyclonal IgG antibody from Antibodies.com targets SNRPD3 in human samples and is validated for Western blot, immunohistochemistry, and flow cytometry workflows.

Provided unconjugated in a 100 µl vial, the reagent supports detection of endogenous SNRPD3 in cell lysates, fixed tissue sections, and prepared cell suspensions. Polyclonal reactivity against multiple epitopes on SNRPD3 helps preserve recognition even when modest structural variation is present, which is useful when probing splicing factor redistribution in nuclear speckles or characterizing autoantibody profiles associated with systemic autoimmune disease research.

For Western blot applications, load cleared cell or nuclear extracts under standard reducing conditions. For IHC, deparaffinized sections typically benefit from citrate-based antigen retrieval with a horseradish peroxidase or fluorescence-labeled secondary reagent. For flow cytometry, fixation and permeabilization are required to access nuclear SNRPD3, with detection through a fluorophore-conjugated secondary antibody. Short-term storage at 4 degrees C works for working aliquots; aliquot and freeze at -20 degrees C for longer-term use to limit freeze-thaw cycles.