Anti-SNCA (phospho Y133) Antibody - Identical to Abcam (ab194910)

Phospho-specific detection of α-synuclein at tyrosine 133 supports studies of Parkinson's disease and related synucleinopathies. This rabbit polyclonal IgG antibody, supplied by Antibodies.com, was raised against a phosphorylated peptide corresponding to the Y133 epitope of human SNCA, allowing selective recognition of the modified residue over the unmodified form. The unconjugated reagent is provided in a 100 µL vial and is validated for Western blot and immunofluorescence workflows, with reactivity confirmed against human, mouse, and rat samples.

In Western blot, the antibody reveals the phosphorylated α-synuclein band against a background of total SNCA expression, supporting time-course and inhibitor studies of tyrosine kinase signaling at this site. For immunofluorescence, the reagent produces granular cytoplasmic staining consistent with synaptic and perinuclear distribution in neurons and transfected cell lines. The polyclonal format broadens epitope coverage so that minor flanking sequence variation across species does not abolish binding, while IgG isotype permits detection with standard anti-rabbit secondary reagents labeled for HRP, fluorophore, or biotin chemistries.

Storage at -20 °C in working aliquots preserves phospho-epitope recognition and limits freeze-thaw damage to the antibody protein. Researchers investigating LRRK2, c-Abl, and Src family kinase pathways, or screening small-molecule modulators that alter Y133 phosphorylation in dopaminergic neurons, use this reagent alongside total α-synuclein antibodies as a phospho/total readout in cell culture and tissue preparations.