Anti-SMO Antibody

Raised in rabbit against the Smoothened (SMO) receptor, this unconjugated polyclonal IgG antibody from Antibodies.com is supplied as 50 µl of ready-to-use reagent and is validated for Western blot and immunohistochemistry workflows. SMO is a Frizzled-class seven-transmembrane G-protein-coupled receptor that transduces Hedgehog pathway signals from the patched co-receptor to GLI transcription factors, controlling embryonic patterning, adult stem cell maintenance, and tissue regeneration. Reactivity studies confirm detection of human, mouse, and rat orthologues, making the reagent useful for comparative studies in developing neural tissue, skin, and tumor xenografts where Hedgehog signaling is active. The unconjugated format allows flexible labelling with HRP, biotin, or fluorophore conjugates of the user's choice, and the polyclonal antibody binds multiple epitopes on SMO, increasing tolerance for minor denaturation or partial proteolysis in fixed samples. Compatible with fresh-frozen and formalin-fixed paraffin-embedded tissue sections, with band sizes consistent with the full-length ~86 kDa Smoothened protein in lysate Western blot.