Anti-SMC4 Antibody

Raised in rabbit against human Structural Maintenance of Chromosomes 4, this unconjugated polyclonal antibody from Antibodies.com detects the 170 kDa condensin core subunit in Western blot and immunohistochemistry workflows. The 100 µL reagent is supplied as an affinity-purified antiserum that recognizes epitopes within the SMC4 polypeptide, which heterodimerizes with SMC2 to form the kleisin-bridged ring that topologically entraps chromatin fibers during mitotic chromosome assembly, sister chromatid resolution, and dosage compensation.

Researchers use this reagent to study chromosome segregation, centromeric organization, DNA double-strand break repair, and condensin dysregulation in hepatocellular carcinoma, colorectal cancer, and acute myeloid leukemia. End-user titration is recommended for both Western blot on whole-cell lysates and immunohistochemistry on FFPE tissue sections, with the unconjugated format allowing choice of secondary reporter.

Storage at -20 °C in working aliquots limits freeze-thaw loss; a glycerol-containing buffer prevents ice crystal damage. Each lot is quality-controlled by Western blot against HeLa nuclear extracts, where the antibody produces a clean band at the expected molecular weight. Intended for research use only and not for diagnostic or therapeutic applications.