Anti-SMC1A (N-terminus) Antibody

This mouse monoclonal antibody from Antibodies.com targets the N-terminus of Structural Maintenance of Chromosomes 1A (SMC1A), a core subunit of the cohesin ring complex that holds sister chromatids together after DNA replication and participates in homologous recombination repair. The reagent is supplied as a 100 µl aliquot of purified immunoglobulin in 0.1 M Tris-Glycine buffer (pH 7.4) containing 150 mM NaCl, 50% glycerol, and 0.2% sodium azide as a preservative, and is unconjugated for direct detection with secondary reagents of choice.

Validated applications include western blot for measuring endogenous cohesin levels in cell extracts, immunohistochemistry on fixed tissue sections to map SMC1A distribution, and flow cytometry for analyzing chromatin-associated protein expression in individual cells. Reactivity is established for human samples, reflecting the conserved role of cohesin in vertebrate genome maintenance and the clinical interest in SMC1A variants linked to Cornelia de Lange syndrome and related cohesinopathies.

The clone recognizes an epitope within the N-terminal region of the protein, which harbors the head domain involved in ATPase-driven heterodimerization with SMC3, making it suitable for studies of cohesin loading onto chromatin, release by WAPL, and the regulatory influence of NIPBL and MAU2. Researchers investigating DNA double-strand break repair, sister chromatid cohesion, or chromatin loop extrusion will find this reagent useful for confirming expression changes following RNAi knockdown, CRISPR editing, or chemical disruption of the cohesin complex, as well as for monitoring the involvement of SMC1A in cell cycle checkpoint signaling.