Anti-SMC 1 Antibody

Raised in rabbits, this polyclonal reagent targets Structural Maintenance of Chromosomes 1 (SMC1), a core subunit of the cohesin ring complex that holds sister chromatids together through S phase and mitosis. Antibodies.com supplies the antibody as a primary reagent at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, providing a glycerol-stabilized buffer suited to routine freeze-thaw cycles when aliquoted at first use. The unconjugated preparation is validated for western blot, immunohistochemistry, and immunofluorescence staining across human, mouse, and rat samples, making it applicable to chromatin biology, cell cycle checkpoint, and DNA damage response research. Investigators commonly use antibodies of this format to visualize cohesin loading at centromeres, follow ATM-dependent phosphorylation of SMC1 after irradiation, and characterize the role of SMC1 in maintaining genome stability under replication stress. The 100 µl vial gives enough volume for multiple staining runs and replicate blots, while the polyclonal mix improves recognition across conserved epitopes of the target—an advantage with primary tissue sections or patient-derived material where epitope accessibility varies. Storage at -20°C in working aliquots is recommended to preserve long-term activity.