Anti-SMARCE1 Antibody - Identical to Abcam (ab186824)

Rabbit polyclonal antibodies raised against the BAF57/SMARCE1 subunit of the SWI/SNF chromatin remodeling complex, manufactured by Antibodies.com to match the ab186824 specification. The 100 µl serum-format IgG reagent is supplied unconjugated, allowing researchers to pair it with HRP or fluorescent secondaries of their choice for chromogenic or fluorescence readouts. It cross-reacts with both human and mouse orthologs of the target and is validated for Western blotting, immunohistochemistry, and immunofluorescence workflows running on whole-cell lysates and formalin-fixed paraffin-embedded tissue sections.

SMARCE1 (also called BAF57) sits within the ATP-dependent SWI/SNF complex that remodels nucleosomes and gates transcription factor access to chromatin. Heterozygous loss-of-function mutations in SMARCE1 cause Coffin-Siris syndrome type 4, a chromatinopathy marked by developmental delay, intellectual disability, and characteristic dysmorphic features, while reduced BAF57 expression has been documented in meningioma and several solid-tumor models where it intersects with tumor suppressor pathways. Within the nucleus, immunofluorescence staining reveals the diffuse chromatin-associated distribution expected of a remodeling complex subunit, and immunoprecipitation from HeLa or HEK293 lysates confirms its association with other SWI/SNF components.

The polyclonal format recognizes multiple epitopes on the 411-amino-acid human protein, improving the chance of detection across splice variants and orthologs. Antibodies.com ships the vial refrigerated at 4 °C; upon arrival users should aliquot and store at -20 °C, avoiding repeated freeze/thaw cycles to preserve affinity-purified IgG activity. For research use only, not intended for diagnostic or therapeutic applications.