Anti-SMARCB1 Antibody

SMARCB1 is a core subunit of the ATP-dependent SWI/SNF chromatin remodeling complex, where it contributes to nucleosome repositioning and transcriptional control. This rabbit polyclonal reagent from Antibodies.com is raised against SMARCB1 (also referenced in the literature as INI1, BAF47, or SNF5), enabling detection across western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, and chromatin immunoprecipitation workflows.

The antibody recognizes endogenous SMARCB1 in human, mouse, and rat samples, supporting comparative oncology, neuroscience, and developmental biology studies. Loss of SMARCB1 expression is the defining molecular event in malignant rhabdoid tumors and atypical teratoid/rhabdoid tumors (AT/RT), and this reagent is used in pathology research to confirm deficient nuclear staining alongside retained positive controls in matched tissue.

Supplied as 100 µl at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, the unconjugated polyclonal preparation allows flexible downstream labeling for fluorescence, HRP, or biotin-based detection systems. Aliquot on receipt and store at -20 degrees C, avoiding repeated freeze-thaw cycles to preserve binding activity. For chromatin immunoprecipitation, the antibody enriches SMARCB1-associated regulatory regions from cross-linked chromatin extracts prepared from cultured mammalian cells.