Anti-SMARCA5 Antibody

This rabbit polyclonal reagent from Antibodies.com targets SMARCA5, the catalytic subunit of the ISWI family chromatin-remodeling complex, also known as SNF2H. The antibody recognizes an epitope conserved across human, mouse, and rat orthologues, supporting comparative work on nucleosome spacing, DNA replication, and chromatin-associated repair pathways.

Validated applications include Western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, and chromatin immunoprecipitation, enabling both protein-level detection and locus-level mapping of SMARCA5 recruitment at specific genomic regions. The unconjugated format is supplied in a 100 µl vial and is ready to be titered into standard chromogenic, fluorescent, or chemiluminescent detection workflows, or to be conjugated as the experimental design requires.

Polyclonal reactivity broadens epitope coverage, which is useful when probing for isoform-specific or post-translationally modified states of SMARCA5 in fractionated chromatin samples. For handling, refrigerate at 4 °C for routine short-term use, and aliquot before freezing at -20 °C for longer-term storage to limit freeze-thaw cycles and preserve binding activity across repeated experiments.