Anti-SMARCA4 Antibody

The SMARCA4 protein (also called BRG1, or Brahma-related gene 1) functions as the catalytic ATPase subunit of the SWI/SNF chromatin-remodeling complex, where it uses ATP hydrolysis to reposition nucleosomes and regulate accessibility of transcription factors to chromatin. Antibodies.com offers this affinity-purified rabbit polyclonal reagent for researchers studying this nuclear ATPase in the context of transcriptional regulation, differentiation, and tumor biology.

This unconjugated antibody recognizes epitopes conserved across human, mouse, and rat SMARCA4, supporting experimental readouts including Western blot (WB) for total protein expression, immunoprecipitation (IP) for complex-member analysis, immunofluorescence (IF) for sub-nuclear localization, and immunohistochemistry (IHC) on formalin-fixed paraffin-embedded tissue.

The reagent is supplied as 100 µl at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2, a buffer composition that preserves antibody activity for routine refrigerated storage. Loss of SMARCA4 expression is a defining diagnostic feature in several malignancies - including rhabdoid tumors, small cell carcinoma of the ovary of the hypercalcemic type (SCCOHT), and a subset of non-small cell lung cancers - making this Antibodies.com reagent useful in research characterizing SWI/SNF-deficient tumors and chromatin remodeling mechanisms.