Anti-SMAD9 Antibody

This unconjugated rabbit polyclonal IgG from Antibodies.com targets the SMAD9 transcription factor, an intracellular mediator of bone morphogenetic protein (BMP) and TGF-beta signaling in human cells. It is supplied as a 100 µl aliquot validated for Western blot and immunofluorescence workflows on human samples, giving laboratories a flexible primary reagent for cell signaling and developmental biology research.

SMAD9 (also referred to as MADH6 or SMAD8 in older literature) sits downstream of type I BMP receptors and translocates to the nucleus upon phosphorylation, where it partners with SMAD4 to regulate transcription of osteogenic, chondrogenic, and vascular gene programs. Researchers use anti-SMAD9 antibodies to study BMP-driven processes including osteoblast and chondrocyte differentiation, pulmonary arterial smooth muscle homeostasis, embryonic patterning, and selected tumor contexts in which BMP signaling has been implicated.

As a polyclonal reagent, the antibody recognizes multiple epitopes on the SMAD9 protein, which is useful when working with truncated isoforms, post-translationally modified protein, or low-abundance targets in fixed cells. The unconjugated format lets users pair the antibody with their own fluorophore- or enzyme-conjugated secondary antibody, supporting both indirect immunofluorescence imaging and chemiluminescent Western blotting. Recommended storage is refrigerated, with handling consistent with standard polyclonal IgG preparations. For research use only.