Anti-SMAD7 Antibody

SMAD7 functions as an inhibitory member of the SMAD family of signal transducers and serves as an intracellular antagonist of transforming growth factor-beta (TGF-β) signaling, providing negative feedback within the receptor cascade. This unconjugated polyclonal reagent is raised in rabbit, purified as IgG, and supplied in a 50 µl vial sized for routine Western blot and immunofluorescence experiments on human samples. Reactive with human SMAD7, the antibody supports research into TGF-β pathway regulation, renal and hepatic fibrosis mechanisms, inflammatory cytokine signaling, and tumor biology, where the protein's role in modulating activated type I receptor complexes is a frequent focus.

For immunoblotting, the expected target band corresponds to SMAD7's reported molecular weight near 46 kDa, while immunofluorescence enables visualization of SMAD7 localization in fixed cell preparations, useful for mapping subcellular distribution under stimulated versus basal signaling conditions. Produced by Antibodies.com as a primary antibody, this reagent is provided unconjugated to allow flexible choice of secondary detection systems, including HRP- or fluorophore-conjugated secondaries matched to the IgG isotype. The antibody is intended for research use only.