Anti-SMAD7 Antibody

This rabbit polyclonal IgG reagent from Antibodies.com recognises SMAD7, an intracellular negative regulator of TGF-beta / BMP receptor signalling that is widely studied in fibrosis, inflammation, and cancer pathway research. The unconjugated antibody is supplied in a 200 µl vial and has been validated for western blot (WB) and immunofluorescence (IF) workflows on human cells and tissue, supporting detection of endogenous SMAD7 in whole-cell lysates, cultured lines, and fixed sections. As a polyclonal reagent raised in rabbit, it provides broad epitope coverage across the SMAD7 protein, and the unconjugated format lets researchers pair it with their own choice of fluorescent, HRP, or biotinylated secondary reagents. Standard western blot protocols include resolving lysates by SDS-PAGE, transferring to nitrocellulose or PVDF membrane, and probing at an optimised dilution for chemiluminescent or near-infrared detection. For immunofluorescence, fixed and permeabilised cells can be stained to visualise subcellular SMAD7 distribution. Store refrigerated and protect from light; for long-term storage, freeze working aliquots at -20 °C and avoid repeated freeze-thaw cycles to maintain antibody activity. For research use only.