Anti-SMAD7 Antibody

This rabbit polyclonal IgG reagent from Antibodies.com binds human SMAD7, the inhibitory SMAD that blocks phosphorylation of receptor-regulated SMADs and recruits ubiquitin ligases to the active TGF-β receptor complex, terminating canonical TGF-β and BMP signaling at the cytoplasmic level. Supplied as 100 µl of unconjugated antiserum, the antibody is ready for use in Western blot and immunofluorescence workflows on human samples, with secondary anti-rabbit reagents providing signal generation.

Endogenous SMAD7 expression is interrogated in epithelial, renal, intestinal, and tumor cell systems where it acts as a feedback brake on TGF-β superfamily signaling. Researchers monitor transcriptional negative-feedback loops after stimulation with ligands such as TGF-β, BMP2/4/7, or activin, and track subcellular partitioning of SMAD7 between the nucleus and cytoplasm under varying signal intensities. The polyclonal format recognizes multiple epitopes across the full-length protein, which is helpful when probing post-translational modifications including ubiquitination, acetylation, and phosphorylation events on the SMAD7 MH2 domain.

For Western blot, the reagent is applied to whole-cell lysates from human cell lines resolved by SDS-PAGE, with chemiluminescent detection via anti-rabbit HRP secondary antibodies. For immunofluorescence, it is used on fixed, permeabilized cells to map SMAD7 distribution before and after ligand treatment and to distinguish receptor-proximal pools from chromatin-associated pools. Store short-term at 4°C; for longer-term stability, aliquot the antiserum and freeze at -20°C, limiting freeze-thaw cycles.

Format: vial of 100 µl. Host: Rabbit. Clonality: Polyclonal. Isotype: IgG. Conjugate: Unconjugated. Target: SMAD7. Reactivity: Human. Applications: WB, IF. Research use only.