Anti-SMAD4 Antibody

From Antibodies.com, this affinity-purified polyclonal reagent is raised in rabbit against a recombinant human SMAD4 immunogen and is intended for the detection of total endogenous SMAD4 protein in research samples. The antibody is supplied as an unconjugated IgG preparation at 1.0 mg/mL in a 50 µL volume, formulated in phosphate-buffered saline at pH 7.4 (without Mg²⁺ and Ca²⁺) with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol as a cryoprotectant. Validated applications include Western blotting and immunohistochemistry, and the reagent has been confirmed to react with human, mouse, and rat samples, making it suitable for cross-species studies of TGF-β/BMP signalling. Because the product is provided lyophilisation-stable in glycerol-containing buffer, it is typically aliquoted and stored frozen at –20 °C to preserve activity across multiple freeze–thaw cycles. Each lot is purified by affinity chromatography against the immunogen to minimise non-specific background, supporting clean detection of the ~70 kDa SMAD4 mediator in whole-cell lysates and fixed tissue sections.