Anti-SMAD4 Antibody

Intended for research use only, this rabbit polyclonal antibody targets SMAD4, a Co-Smad transcription factor that mediates canonical TGF-β signaling. Produced by Antibodies.com, the reagent is affinity-purified and supplied in a single 100 µL vial of unconjugated antiserum, ready for direct labeling or pairing with a chosen secondary antibody.

Validated for western blot, immunohistochemistry, and immunofluorescence workflows, the antibody has been confirmed to recognize SMAD4 in human, mouse, and rat samples. Polyclonal recognition across multiple epitopes is advantageous for detecting splice variants, truncated mutants, and the wild-type protein alike, supporting studies of pancreatic ductal carcinoma and other TGF-β-driven malignancies where SMAD4 loss is a frequent molecular event.

For best results, aliquot the antibody upon arrival to limit freeze-thaw cycles and store frozen at -20 °C in working volumes. The unconjugated format provides flexibility when working with challenging tissue targets or when paired fluorophore and enzyme conjugates need to be selected for a specific detection platform.