Anti-Smad3 (phospho-S204) Antibody

Antibodies.com offers a rabbit polyclonal antibody raised against Smad3 phosphorylated at serine 204 (phospho-S204), a modification site linked to non-canonical TGF-β signaling and cross-talk with receptor tyrosine kinase pathways. The reagent is supplied at 1 mg/ml in phosphate buffered saline (PBS) with 15 mM sodium azide at approximately pH 7.2, and ships as a 100 µl vial suitable for multiple experiments. For research use only.

This unconjugated primary antibody is validated for western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), and immunoprecipitation (IP), giving laboratories flexibility across sample types. Reactivity has been confirmed against human, mouse, and rat samples, making it a useful reagent for translational studies that compare Smad3 activation across species and tissue contexts.

Phosphorylation of Smad3 at S204 is distinct from the canonical C-terminal phosphorylation at S423/S425 and has been reported in contexts including fibroblast activation, hepatic injury responses, and certain cancer cell models. Researchers studying signaling dynamics, pathway cross-talk, or phosphorylation-specific protein interactions can use this antibody to probe the spatial and temporal pattern of S204 phosphorylation. For long-term use, aliquot and freeze to limit repeated freeze-thaw cycles and preserve antibody activity.