Anti-Smad3 (N175) Antibody

The Smad3 transcription factor sits at the heart of canonical TGF-β signaling, where it partners with Smad4 to translocate into the nucleus and regulate genes involved in fibrosis, epithelial-mesenchymal transition, and inflammatory responses. This rabbit polyclonal reagent, raised against the N-terminal epitope spanning residue 175, recognizes the MH1 DNA-binding domain rather than the receptor-phosphorylated C-terminal tail, so it reports total Smad3 protein levels across western blot and immunohistochemistry workflows.

From Antibodies.com, the unconjugated antibody is validated on human, mouse, and rat samples and supplied at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide at approximately pH 7.2. The unconjugated format fits standard HRP- or fluorophore-labeled secondary detection schemes, and the 50 µl vial suits exploratory blots and limited tissue-section panels in signaling, oncology, and fibrosis research.