Anti-Smad3 Antibody

SMAD family member 3 is a downstream effector within the TGF-β signaling cascade, conveying receptor-derived signals from the cell surface to the nucleus where it modulates transcription of target genes. Antibodies.com provides this affinity-purified polyclonal reagent raised against Smad3 in unconjugated format at 100 µl volume, allowing investigators to pair it with their preferred secondary detection chemistry. It has been validated for Western blot, immunohistochemistry, and ELISA workflows in human, mouse, and rat samples, supporting cross-species comparison in fibrosis models, tumor biology, and developmental signaling research. Typical experimental uses include tracking phosphorylation-dependent activation during epithelial-mesenchymal transition, examining target distribution in renal and pulmonary fibrosis tissue, and probing the dual tumor suppressor and oncogenic roles of the TGF-β pathway. The polyclonal backbone recognizes multiple Smad3 epitopes, increasing tolerance for minor variations in fixation or lysis conditions and yielding cleaner band resolution at the expected molecular weight position, as well as specific cytoplasmic and nuclear staining patterns in formalin-fixed tissue sections. Researchers focused on EMT, organ fibrosis, or pathway crosstalk will find this reagent applicable to standard and exploratory protocols, including co-immunoprecipitation and chromatin-related assays.