Anti-Smad3 (Ab-204) Antibody

Raised in rabbit against a peptide antigen derived from human SMAD3, this polyclonal primary antibody binds the SMAD3 transcription factor for signaling studies. SMAD3 is a key effector of canonical TGF-β signal transduction, relaying receptor activation into changes in target gene expression across multiple tissue types and disease models.

From Antibodies.com, the reagent is supplied as 100 µl of unconjugated antiserum in a single-use vial. It is validated for western blot and immunohistochemistry on Human, Mouse, and Rat samples, enabling detection of endogenous SMAD3 protein in cell lysates, fresh-frozen sections, and formalin-fixed paraffin-embedded tissue.

Recommended for research use only, the antibody fits laboratory workflows profiling TGF-β pathway activity, fibrosis models, epithelial-mesenchymal transition research, and downstream transcriptional responses in cancer biology. Aliquoting upon first use is advised to limit repeated freeze-thaw cycles of the working stock.