Anti-SMAD2 (phospho T220) Antibody

Phosphorylation at threonine 220 of the SMAD2 transcription factor is a key readout of active TGF-β receptor signaling, and this rabbit polyclonal reagent from Antibodies.com is raised specifically against that phosphoepitope. The antibody is supplied as an unconjugated IgG in a 200 µL volume, intended for Western blot detection of human SMAD2 phosphorylated at T220 in cell lysates and tissue extracts.

The polyclonal format means the reagent recognizes multiple epitopes on the phospho-T220 region, which can improve signal in WB when the target is expressed at moderate levels. Reactivity is reported for human samples, and the host species is rabbit, allowing detection using standard anti-rabbit IgG secondary antibodies conjugated to HRP or fluorophores. The product is classified for research use only.

Typical use begins with SDS-PAGE separation of lysate, transfer to nitrocellulose or PVDF, blocking, overnight incubation with the primary antibody at an empirically optimized dilution, and development via chemiluminescence or fluorescence. The phospho-T220 signal can be compared to total SMAD2 levels on a parallel blot to assess pathway activation state in response to TGF-β stimulation or inhibitor treatment.