Anti-Smad2 Antibody

Manufactured by Antibodies.com, this affinity-purified reagent is raised in rabbit and is offered as a polyclonal antiserum specific for endogenous Smad2 protein. The antibody is supplied as a 50 µl liquid formulation at 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, and is unconjugated, making it well suited to standard immunodetection workflows. It was generated against a synthetic peptide derived from human Smad2 surrounding the non-phosphorylation site at threonine 220, and it has been validated for ELISA, Western blot (WB), immunocytochemistry/immunofluorescence (ICC/IF) and immunohistochemistry (IHC). The reagent cross-reacts with human, mouse and rat samples, giving it broad utility across cell-line, primary-tissue and animal-model studies of TGF-β/Smad signaling. For routine handling the supplier recommends shipping at 4 °C followed by aliquoting and storage at −20 °C, with repeated freeze/thaw cycles avoided to preserve activity. Each lot is affinity-purified by epitope-specific chromatography to ensure consistent specificity and low background, and the product is intended for research use only. Researchers investigating Smad2 phosphorylation status, transcriptional regulation or pathway activation will find this polyclonal antibody a reliable choice for sensitive and reproducible detection across multiple assay platforms.