Anti-Smad2 Antibody

The Smad2 protein is a central intracellular effector of the TGF-β signaling pathway, transmitting signals from activated type I serine/threonine kinase receptors to the nucleus, where it regulates transcription of target genes involved in proliferation, differentiation, and extracellular matrix production. Phosphorylation at its C-terminal SSXS motif triggers homotrimerization with Smad4 and nuclear translocation, making activated Smad2 a widely used readout of pathway engagement in cell biology research.

This rabbit polyclonal reagent from Antibodies.com is supplied as an unconjugated liquid in 100 µl, allowing laboratories to pair it with HRP, fluorophore, or biotin detection chemistries of their own choosing. The reagent is reactive with Human, Mouse, and Rat samples and has been validated for western blot, immunohistochemistry, immunofluorescence, and ELISA workflows. It supports research programs investigating fibrosis, epithelial-mesenchymal transition, cancer cell signaling, and developmental biology where canonical TGF-β responses are being characterized.

Because the reagent is polyclonal rather than monoclonal, it engages multiple epitopes across Smad2 rather than a single linear motif, which is helpful when probing both basal and activated pools of the protein in the same experiment. Researchers commonly deploy this reagent on cell culture lysates and on fixed tissue sections to monitor endogenous Smad2 distribution and to track pathway activation in response to TGF-β stimulation.