Anti-Smad2 Antibody

The affinity-purified reagent listed under catalogue number A34456 is a rabbit polyclonal IgG preparation raised against a synthetic peptide derived from the human Smad2 sequence flanking the non-phosphorylation site at S467, allowing it to recognize endogenous levels of the Smad2 protein in human, mouse and rat samples. Distributed by Antibodies.com, this unconjugated primary antibody is supplied as a 50 µL liquid formulation in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide at a working concentration of 1 mg/mL, and it is shipped at 4 °C for short-term stability before long-term storage at –20 °C in single-use aliquots to avoid repeated freeze/thaw cycles. Researchers can apply the antibody across a broad panel of immunoassays, including Western blotting, immunohistochemistry, immunocytochemistry/immunofluorescence and ELISA, with representative validation data from Western blot analysis of COLO cell lysates shown on the product page. The polyclonal format, raised in rabbit and affinity-purified by epitope-specific chromatography, provides multi-epitope coverage of Smad2 (also known as JV18-1, MADH2 or MADR2), making it well suited for studies of TGF-β/BMP signalling, transcriptional regulation by Smad complexes and downstream effects on cell proliferation and differentiation in cell-line and tissue models.