Anti-Smad2 Antibody

From Antibodies.com, this 50 µL vial of purified mouse monoclonal antibody is raised against human Smad2 (SMAD Family Member 2), a key intracellular mediator of TGF-β signalling. The unconjugated reagent is supplied as a purified IgG preparation in a buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% sodium azide and 50% glycerol, and is recommended for Western Blotting (WB) applications. The antibody detects endogenous levels of SMAD2 protein in human samples and is intended for research use only. For routine handling, the glycerol-containing formulation should be stored at –20 °C (with short-term storage at 4 °C for working aliquots), avoiding repeated freeze–thaw cycles to preserve activity. The product is offered by Antibodies.com under their Primary Antibodies catalogue, and the supplied 50 µL pack size is sufficient for multiple standard immunoblotting experiments. Researchers studying TGF-β pathway activity, transcriptional regulation by SMAD complexes, or downstream readouts of receptor serine/threonine kinase signalling will find this reagent a well-defined tool for detecting endogenous SMAD2 in whole-cell lysates.