Anti-Smad2 (Ab-467) Antibody

Antibodies.com presents a rabbit polyclonal antibody raised against Smad2 phosphorylated at residue 467, a regulatory site within the MH2 domain of this TGF-β pathway transcription factor. Phosphorylation of Smad2 at Ser465/Ser467 by the type I TGF-β receptor triggers complex formation with Smad4 and subsequent nuclear translocation, making post-translational modification at this residue a widely used readout of active TGF-β signaling in cell biology and disease research.

This reagent is validated for Western blot detection of phospho-Smad2 levels in cell lysates and for immunofluorescence localization of activated Smad2 in cultured cells and tissue sections. The antibody shows reactivity with Human, Mouse, and Rat samples, supporting translational studies across common model systems. Supplied as 100 µl of unconjugated polyclonal reagent enabling downstream HRP- or fluorophore-conjugated secondary detection.

For storage, aliquot the antibody upon receipt and store at -20°C to preserve phospho-epitope recognition; avoid repeated freeze-thaw cycles. Each batch is affinity-purified and quality-tested for phospho-specific binding to minimize cross-reactivity with the non-phosphorylated form of Smad2.