Anti-Smad2 (Ab-220) Antibody

Manufactured by Antibodies.com, this rabbit polyclonal reagent targets Smad2 at the Ab-220 epitope and is supplied as a 50 µl aliquot at 1.0 mg/mL in phosphate-buffered saline (pH 7.4) with 150 mM NaCl, 0.02% sodium azide and 50% glycerol. The antibody was affinity-purified from rabbit antiserum by epitope-specific affinity chromatography and is provided in unconjugated form, allowing users to choose their own label or detection chemistry. It is validated for western blotting (WB) and immunohistochemistry (IHC) applications and has been confirmed to react with human, mouse and rat samples, detecting endogenous levels of total Smad2 protein. The immunogen is a synthetic non-phosphopeptide corresponding to the region around threonine 220 (P-E-T-P-P) of human Smad2, which is useful for researchers studying TGF-β/Smad signalling, receptor-regulated Smad phosphorylation status and downstream transcriptional responses. As a polyclonal host-rabbit reagent, the product offers broad epitope coverage and is well suited to studies where recognition of multiple Smad2 isoforms or conformers is advantageous. The concentrate is intended for research use only and is not validated for diagnostic or therapeutic applications. Orders are dispatched from the supplier's Cambridge (UK) warehouse with a typical lead time of four to six business days, and the antibody pairs with standard chemiluminescent, fluorescent or chromogenic detection workflows depending on the secondary reagent selected.