Anti-Smad2 (Ab-220) Antibody

Smad2 is a key intracellular signal transducer in the TGF-β (transforming growth factor-beta) pathway, conveying signals from activated cell-surface receptors to the nucleus to regulate transcription of target genes involved in proliferation, differentiation, and extracellular matrix production. This rabbit polyclonal antibody from Antibodies.com was raised against an epitope in the Smad2 protein and is supplied as an unconjugated reagent in a 100 µl volume, providing flexibility for both Western Blot and Immunohistochemistry workflows. It cross-reacts with Human, Mouse, and Rat samples, making it useful for comparative studies across model systems and human tissue. In Western Blot, the reagent detects endogenous Smad2, including phosphorylated forms that report on active TGF-β signaling, while in Immunohistochemistry it supports localization studies in fixed tissue sections to map Smad2 distribution in normal, fibrotic, and tumor specimens. The unconjugated format allows researchers to choose their preferred secondary detection system and to pair the reagent with phospho-specific or total-protein detection strategies as needed for pathway analysis.