Anti-SMAD2/3 (Acetyl-K19) Antibody

Selective detection of the acetyl-lysine 19 mark on SMAD2 and SMAD3 supports research into TGF-β-driven transcriptional control and receptor-regulated signaling cascades. This unconjugated rabbit polyclonal reagent from Antibodies.com is supplied at 1 mg/ml in phosphate buffered saline containing 0.05% sodium azide at approximately pH 7.3, and is validated for Western blot analysis of human, mouse, and rat samples.

The 100 µl vial format provides ample material for multiple immunoblotting experiments probing endogenous levels of modified SMAD proteins in cell lysates and tissue extracts. Aliquot upon receipt and store at 4 °C short term or -20 °C long term to preserve antibody activity across repeated freeze-thaw cycles.

Researchers studying SMAD-mediated transcriptional responses, BMP signaling crosstalk, or histone acetyltransferase involvement in SMAD-dependent gene expression can use this reagent to distinguish acetylated SMAD2/3 species from unmodified protein in Western blot and immunoprecipitation workflows.