Anti-SMAD1 (phospho S465) Antibody

Detection of activated BMP signaling requires reagents that distinguish phosphorylated from resting SMAD1, and this rabbit polyclonal IgG preparation from Antibodies.com selectively binds SMAD1 only when serine 465 is phosphorylated by BMP type I kinases.

Supplied as 50 µl of unconjugated antiserum, the antibody has been validated for Western blot use on human, mouse, and rat samples. Researchers studying osteoblast differentiation, chondrogenesis, neural stem cell fate decisions, hepcidin-mediated iron homeostasis, and BMP-driven tumor suppression rely on S465 phosphorylation as a direct readout of receptor activation.

For Western blot, the antibody is paired with standard anti-rabbit HRP-conjugated secondary reagents and chemiluminescent detection. Recommended storage at -20 degrees C in single-use aliquots preserves phospho-epitope recognition across freeze-thaw cycles, and the unconjugated format offers flexible downstream labelling.