Anti-SMAD1 (phospho S465) Antibody

Produced in rabbit as a polyclonal IgG, this unconjugated antibody from Antibodies.com selectively detects SMAD1 when phosphorylated at serine 465, the BMP type I receptor-driven activation mark that commits SMAD1 to nuclear translocation and target gene transcription in the canonical bone morphogenetic protein pathway. Recommended for Western blot workflows on lysates from human, mouse, and rat samples, the reagent is provided as a 100 µl liquid aliquot and supports BMP-driven osteogenesis, chondrogenesis, and stem cell differentiation studies where phospho-SMAD1 readouts distinguish active signaling from total protein levels. Aliquot upon receipt and store at -20 °C in single-use portions to preserve phospho-specific binding across freeze-thaw cycles; for short-term use, keep refrigerated and protect from light. The unconjugated format offers downstream flexibility, allowing the primary to be paired with HRP- or fluorophore-conjugated secondary antibodies matched to the rabbit host and the chosen Western blot detection chemistry.