Anti-Smad1 (Ab-465) Antibody

From Antibodies.com, this rabbit polyclonal reagent is raised against a peptide sequence surrounding amino acids 461–465 (I-S-S-V-S) of human Smad1 and is supplied as an affinity-purified, unconjugated IgG preparation at 1.0 mg/mL. The 50 µL vial is formulated in phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol, and the antibody is shipped at 4 °C with a recommendation to aliquot and store at –20 °C, avoiding repeated freeze/thaw cycles. It is validated for Western blot and immunocytochemistry/immunofluorescence (ICC/IF) and detects the endogenous level of total Smad1 (~55 kDa) in human, mouse, and rat samples. Western blot analysis of HeLa cell extracts and immunofluorescence staining of methanol-fixed HeLa cells are provided as representative data, supporting use across signaling, developmental, and BMP-pathway research applications.