Anti-Smad1 (Ab-465) Antibody

This rabbit polyclonal antibody from Antibodies.com specifically recognizes Smad1, a receptor-regulated intracellular transducer of the bone morphogenetic protein (BMP) branch of the TGF-β signaling pathway. Smad1 is phosphorylated at its C-terminal SSXS motif by BMP type I receptors, then partners with Smad4 and accumulates in the nucleus to regulate transcription of osteogenic, chondrogenic, and developmental target genes.

Supplied as 100 µL of unconjugated antiserum, the reagent is validated for Western blot and immunofluorescence workflows. It cross-reacts with Human, Mouse, and Rat samples, supporting comparative studies across common model systems and primary cells. For Western blot, separated proteins are transferred to a membrane and probed under standard reducing conditions; for immunofluorescence, the antibody is applied to fixed cells to track Smad1 distribution and BMP-driven nuclear translocation following ligand stimulation.

Storage recommendations follow standard polyclonal antibody handling: aliquot the vial upon arrival, store frozen at -20 °C in working volumes to limit freeze-thaw cycles, and protect from light. The reagent is intended for research use only and has not been validated for diagnostic or therapeutic procedures.