Anti-Smad1 (Ab-465) Antibody

Raised in rabbit against a region centered around residue 465 of human Smad1, this unconjugated polyclonal primary antibody from Antibodies.com is validated for western blot (WB) and immunohistochemistry (IHC). Smad1 operates as a receptor-regulated mediator in the BMP branch of the TGF-β pathway; phosphorylation of its C-terminal SSXS motif by BMP type I receptors triggers nuclear translocation and drives downstream transcription of osteogenic, chondrogenic, and developmental gene programs.

Supplied as 100 µL of liquid stock, the reagent offers polyclonal epitope coverage enabling detection of both unmodified and phosphorylated Smad1 species. Pair the antibody with HRP-, biotin-, or fluorophore-conjugated secondary reagents depending on the readout, applying it in standard reducing SDS-PAGE immunoblotting as well as fixed-section IHC on human tissue specimens.

Reactivity with human Smad1 makes the reagent appropriate for BMP pathway profiling, bone morphogenetic protein response studies in cell culture models, and broader signal transduction research. For best long-term stability, aliquot upon arrival and store frozen at -20 °C, avoiding repeated freeze-thaw cycles; working aliquots kept refrigerated are stable for routine blotting sessions across several weeks.