Anti-Smad1 / 5 / 9 Antibody

This polyclonal reagent from Antibodies.com is raised in rabbit against the conserved MH2-domain epitope shared by Smad1, Smad5, and Smad9, three receptor-regulated Smad proteins that mediate bone morphogenetic protein (BMP) signaling. The unconjugated antibody is supplied as 100 µl of antiserum and is validated for western blot, immunohistochemistry, and ELISA applications on human, mouse, and rat samples.

Because Smad1, Smad5, and Smad9 are highly homologous within their C-terminal phospho-acceptor regions, a polyclonal reagent commonly detects all three receptor-regulated forms in parallel, including both basal and BMP-induced phosphorylated species, simplifying pathway-level studies in a single blot or tissue section. Typical research uses include profiling osteogenic and chondrogenic differentiation, examining dorsal-ventral axis patterning in developmental models, and mapping crosstalk between BMP and TGF-β signaling cascades. To preserve binding activity across multiple experiments, aliquot the antiserum on arrival and limit repeated freeze-thaw cycles of the working stock.