Anti-SLC6A1 Antibody

A rabbit polyclonal IgG reagent from Antibodies.com raised against the GABA transporter 1 (GAT-1, encoded by SLC6A1), supplied as 200 µl of unconjugated, affinity-purified antibody validated for western blot and immunohistochemistry on human, mouse, and rat samples. The target runs as an ~67 kDa band in tissue lysates, and the antibody produces clear cytoplasmic and neuropil staining in fixed sections of cortex, hippocampus, and cerebellum, making it a routine marker for inhibitory GABAergic neurons. Researchers use this reagent to chart GAT-1 distribution in rodent disease models, to characterize loss-of-function SLC6A1 variants linked to myoclonic-atonic epilepsy and autism spectrum disorder, and to map inhibitory circuitry in neurodevelopmental studies. Each vial of antiserum is aliquoted in a PBS-based cryoprotective buffer and stored frozen at -20 °C to preserve activity across repeated working-aliquot cycles, with brief thawing on ice recommended immediately before use. Working dilutions in published protocols commonly fall in the 1:500–1:1000 range for western blot and 1:100–1:500 for immunohistochemistry, though end-users should titrate for their specific substrate and detection system. The unconjugated format allows pairing with any fluorophore- or enzyme-labeled secondary antibody, supporting both chromogenic and fluorescence-based readouts on membranes and fixed tissue sections.