Anti-SLC22A6 Antibody

Renal drug clearance research depends on sensitive detection of organic anion transporters, and this rabbit polyclonal reagent against the SLC22A6 carrier from Antibodies.com is purpose-built for that application. The antibody targets the basolateral membrane protein that initiates urinary excretion in proximal tubule cells, and it recognizes both human and rat orthologs, supporting translational studies that span rodent disease models and human tissue.

Each vial provides 100 µl of unconjugated IgG ready for Western blot workflows on whole-cell lysates, kidney cortex homogenates, and membrane fractions separated under denaturing SDS-PAGE. Typical published use involves overnight primary incubation at 4 °C followed by HRP-conjugated or fluorescent secondary detection; titrate against a positive control such as human kidney cortex lysate or an OAT1-overexpressing cell line to determine the working dilution for your system.

Aliquot upon first thaw, refrigerate the working stock, and avoid repeated freeze-thaw cycles to preserve antibody titer across long-running expression studies. The reagent is widely used to investigate drug-drug interactions involving methotrexate, certain cephalosporins, and loop and thiazide diuretics that share the OAT1 transport pathway, making it a practical tool for nephrotoxicity screening and pharmacokinetic profiling.