Anti-SKAP2 Antibody

Raised in rabbits and supplied as an unconjugated polyclonal reagent, this Antibodies.com antibody targets SKAP2 (Src Kinase-Associated Phosphoprotein 2), a cytosolic adapter that participates in immune receptor signaling, integrin-mediated adhesion, and cytoskeletal remodeling across T-cell, B-cell, and myeloid lineages. The 100 µl volume is validated for Western blot (WB) and immunofluorescence (IF) workflows on human-derived samples, supporting detection of endogenous protein in cell lysates and fixed-cell preparations.

SKAP2 carries a pleckstrin homology domain and an SH3 domain that mediate interactions with partners such as FYB, SKAP1, and SLAP-130, positioning the protein downstream of Src-family kinases and upstream of Rap1 during LFA-1 activation. Research applications include profiling T-cell and B-cell receptor signaling, mapping integrin inside-out signaling, and examining macrophage adhesion. The polyclonal format offers broader epitope coverage for tracking endogenous expression, splice variants, and post-translational modifications under varied experimental conditions.

For handling, aliquot upon receipt and store at -20 °C, avoiding repeated freeze-thaw cycles to preserve binding activity. Protect from light when not in use. For research use only.